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cd45 buv395  (Miltenyi Biotec)


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    Miltenyi Biotec cd45 buv395
    (A) Flow cytometry analysis of % immune cells present in LPM whole mounts between mock- and GBS-infected group. (B) % LPM CD206 + /Lyve1 + cells of live <t>CD45</t> + immune cells and (C) Lyve1 mean fluorescence intensity of cells in mock- and GBS-infected group from (A). (n=5 mock, n=4 GBS for 3A-3C) (D) Representative images of L-BAMs using IF on LPM whole mounts. (E) Total CD206 + cell count and (F) Lyve1 intensity per CD206 + /Lyve1 + cell (CTCF) from (D) . (n=7 mock, n=4 GBS for 3E and 3F) Statistics: Mann-Whitney U test, * = p < 0.05, ** = p < 0.01, ns = not significant; mean and SD. Scale bar = (D) 20µm.
    Cd45 Buv395, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd45+buv395/TCR+V%CE%B213%2E1+Antibody%2C+anti-human%2C+REAfinity/bio_rxiv__64898__2026__03__04__709573-177-39-58
    Average 94 stars, based on 4 article reviews
    cd45 buv395 - by Bioz Stars, 2026-10
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    Images

    1) Product Images from "Meningeal inflammation and arachnoid barrier breakdown in a mouse model of neonatal bacterial meningitis"

    Article Title: Meningeal inflammation and arachnoid barrier breakdown in a mouse model of neonatal bacterial meningitis

    Journal: bioRxiv

    doi: 10.64898/2026.03.04.709573

    (A) Flow cytometry analysis of % immune cells present in LPM whole mounts between mock- and GBS-infected group. (B) % LPM CD206 + /Lyve1 + cells of live CD45 + immune cells and (C) Lyve1 mean fluorescence intensity of cells in mock- and GBS-infected group from (A). (n=5 mock, n=4 GBS for 3A-3C) (D) Representative images of L-BAMs using IF on LPM whole mounts. (E) Total CD206 + cell count and (F) Lyve1 intensity per CD206 + /Lyve1 + cell (CTCF) from (D) . (n=7 mock, n=4 GBS for 3E and 3F) Statistics: Mann-Whitney U test, * = p < 0.05, ** = p < 0.01, ns = not significant; mean and SD. Scale bar = (D) 20µm.
    Figure Legend Snippet: (A) Flow cytometry analysis of % immune cells present in LPM whole mounts between mock- and GBS-infected group. (B) % LPM CD206 + /Lyve1 + cells of live CD45 + immune cells and (C) Lyve1 mean fluorescence intensity of cells in mock- and GBS-infected group from (A). (n=5 mock, n=4 GBS for 3A-3C) (D) Representative images of L-BAMs using IF on LPM whole mounts. (E) Total CD206 + cell count and (F) Lyve1 intensity per CD206 + /Lyve1 + cell (CTCF) from (D) . (n=7 mock, n=4 GBS for 3E and 3F) Statistics: Mann-Whitney U test, * = p < 0.05, ** = p < 0.01, ns = not significant; mean and SD. Scale bar = (D) 20µm.

    Techniques Used: Flow Cytometry, Infection, Fluorescence, Cell Characterization, MANN-WHITNEY

    Related Articles

    Staining:

    Article Title: Meningeal inflammation and arachnoid barrier breakdown in a mouse model of neonatal bacterial meningitis
    Article Snippet: Single cell suspensions were first stained with eBioscience Fixable Viability Dye eFluor 506 (Catalog # 65-0866-18) in PBS for 30 minutes at room temperature. .. Cells were stained with the following anti-mouse surface antibodies in MACS buffer for 30 minutes at room temperature: from BioLegend—F4/80-BV785 (clone BM8; Catalog #123141), Ultra-LEAF Purified CD16/32 (clone 93; Catalog # 101330]; from BDBiosciences—CD19-PE-Cy5 (clone 1D3; Catalog # 558079), CD45-BUV395 (clone 30-F11; Catalog # 564279), IA/IE-AF700 (clone M5/114.15.2; Catalog # 748845), Ly6C-PercP (clone AL-21; Catalog # 562727); from Miltenyi Biotec—CD11b-APCVio 770 (clone M1/70; Catalog # 11-0112-41); from ThermoFisher Invitrogen—CD206-PE-Cy7 (clone 1A8-Ly6g; Catalog # 17-9668-82), Ly6G-APC (clone 1A8-Ly6g; Catalog # 17-9668-82), Lyve1-PE (clone 1A8-Ly6g; Catalog # 17-9668-82), P2RY12-AF488 (clone 1A8-Ly6g; Catalog # 17-9668-82). .. After surface antibody staining, the cells were fixed for 30 minutes at room temperature using the FoxP3 fixation/permeabilization kit (ThermoFisher Scientific, Catalog # 00-5523-00).

    Magnetic Cell Separation:

    Article Title: Meningeal inflammation and arachnoid barrier breakdown in a mouse model of neonatal bacterial meningitis
    Article Snippet: Single cell suspensions were first stained with eBioscience Fixable Viability Dye eFluor 506 (Catalog # 65-0866-18) in PBS for 30 minutes at room temperature. .. Cells were stained with the following anti-mouse surface antibodies in MACS buffer for 30 minutes at room temperature: from BioLegend—F4/80-BV785 (clone BM8; Catalog #123141), Ultra-LEAF Purified CD16/32 (clone 93; Catalog # 101330]; from BDBiosciences—CD19-PE-Cy5 (clone 1D3; Catalog # 558079), CD45-BUV395 (clone 30-F11; Catalog # 564279), IA/IE-AF700 (clone M5/114.15.2; Catalog # 748845), Ly6C-PercP (clone AL-21; Catalog # 562727); from Miltenyi Biotec—CD11b-APCVio 770 (clone M1/70; Catalog # 11-0112-41); from ThermoFisher Invitrogen—CD206-PE-Cy7 (clone 1A8-Ly6g; Catalog # 17-9668-82), Ly6G-APC (clone 1A8-Ly6g; Catalog # 17-9668-82), Lyve1-PE (clone 1A8-Ly6g; Catalog # 17-9668-82), P2RY12-AF488 (clone 1A8-Ly6g; Catalog # 17-9668-82). .. After surface antibody staining, the cells were fixed for 30 minutes at room temperature using the FoxP3 fixation/permeabilization kit (ThermoFisher Scientific, Catalog # 00-5523-00).

    Purification:

    Article Title: Meningeal inflammation and arachnoid barrier breakdown in a mouse model of neonatal bacterial meningitis
    Article Snippet: Single cell suspensions were first stained with eBioscience Fixable Viability Dye eFluor 506 (Catalog # 65-0866-18) in PBS for 30 minutes at room temperature. .. Cells were stained with the following anti-mouse surface antibodies in MACS buffer for 30 minutes at room temperature: from BioLegend—F4/80-BV785 (clone BM8; Catalog #123141), Ultra-LEAF Purified CD16/32 (clone 93; Catalog # 101330]; from BDBiosciences—CD19-PE-Cy5 (clone 1D3; Catalog # 558079), CD45-BUV395 (clone 30-F11; Catalog # 564279), IA/IE-AF700 (clone M5/114.15.2; Catalog # 748845), Ly6C-PercP (clone AL-21; Catalog # 562727); from Miltenyi Biotec—CD11b-APCVio 770 (clone M1/70; Catalog # 11-0112-41); from ThermoFisher Invitrogen—CD206-PE-Cy7 (clone 1A8-Ly6g; Catalog # 17-9668-82), Ly6G-APC (clone 1A8-Ly6g; Catalog # 17-9668-82), Lyve1-PE (clone 1A8-Ly6g; Catalog # 17-9668-82), P2RY12-AF488 (clone 1A8-Ly6g; Catalog # 17-9668-82). .. After surface antibody staining, the cells were fixed for 30 minutes at room temperature using the FoxP3 fixation/permeabilization kit (ThermoFisher Scientific, Catalog # 00-5523-00).



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    Image Search Results


    (A) Flow cytometry analysis of % immune cells present in LPM whole mounts between mock- and GBS-infected group. (B) % LPM CD206 + /Lyve1 + cells of live CD45 + immune cells and (C) Lyve1 mean fluorescence intensity of cells in mock- and GBS-infected group from (A). (n=5 mock, n=4 GBS for 3A-3C) (D) Representative images of L-BAMs using IF on LPM whole mounts. (E) Total CD206 + cell count and (F) Lyve1 intensity per CD206 + /Lyve1 + cell (CTCF) from (D) . (n=7 mock, n=4 GBS for 3E and 3F) Statistics: Mann-Whitney U test, * = p < 0.05, ** = p < 0.01, ns = not significant; mean and SD. Scale bar = (D) 20µm.

    Journal: bioRxiv

    Article Title: Meningeal inflammation and arachnoid barrier breakdown in a mouse model of neonatal bacterial meningitis

    doi: 10.64898/2026.03.04.709573

    Figure Lengend Snippet: (A) Flow cytometry analysis of % immune cells present in LPM whole mounts between mock- and GBS-infected group. (B) % LPM CD206 + /Lyve1 + cells of live CD45 + immune cells and (C) Lyve1 mean fluorescence intensity of cells in mock- and GBS-infected group from (A). (n=5 mock, n=4 GBS for 3A-3C) (D) Representative images of L-BAMs using IF on LPM whole mounts. (E) Total CD206 + cell count and (F) Lyve1 intensity per CD206 + /Lyve1 + cell (CTCF) from (D) . (n=7 mock, n=4 GBS for 3E and 3F) Statistics: Mann-Whitney U test, * = p < 0.05, ** = p < 0.01, ns = not significant; mean and SD. Scale bar = (D) 20µm.

    Article Snippet: Cells were stained with the following anti-mouse surface antibodies in MACS buffer for 30 minutes at room temperature: from BioLegend—F4/80-BV785 (clone BM8; Catalog #123141), Ultra-LEAF Purified CD16/32 (clone 93; Catalog # 101330]; from BDBiosciences—CD19-PE-Cy5 (clone 1D3; Catalog # 558079), CD45-BUV395 (clone 30-F11; Catalog # 564279), IA/IE-AF700 (clone M5/114.15.2; Catalog # 748845), Ly6C-PercP (clone AL-21; Catalog # 562727); from Miltenyi Biotec—CD11b-APCVio 770 (clone M1/70; Catalog # 11-0112-41); from ThermoFisher Invitrogen—CD206-PE-Cy7 (clone 1A8-Ly6g; Catalog # 17-9668-82), Ly6G-APC (clone 1A8-Ly6g; Catalog # 17-9668-82), Lyve1-PE (clone 1A8-Ly6g; Catalog # 17-9668-82), P2RY12-AF488 (clone 1A8-Ly6g; Catalog # 17-9668-82).

    Techniques: Flow Cytometry, Infection, Fluorescence, Cell Characterization, MANN-WHITNEY

    Flow Cytometry ICS targeted T cell panel detailing antibody fluorochrome, channel, clone, sources and staining.

    Journal: ImmunoHorizons

    Article Title: Comprehensive immune profiling of dengue and chikungunya viral responses using a novel miniaturized automated whole blood cellular analysis system and mass cytometry in a pediatric cohort in Msambweni, Kenya

    doi: 10.1093/immhor/vlaf006

    Figure Lengend Snippet: Flow Cytometry ICS targeted T cell panel detailing antibody fluorochrome, channel, clone, sources and staining.

    Article Snippet: BUV395 , UVG , CD45 , HI30 , FISHER SCIENTIFIC , Surface , 5.

    Techniques: Flow Cytometry, Staining

    Alteration of the microbiota-regulated metabolic profile is linked to immune dysregulation in RRMS (A) Percentage of circulating T cell subsets in RRMS patients and HCs measured by multiparametric flow cytometry. γδT cells were identified as CD45 + CD3 + TCRγδ + cells; MAIT cells were defined as CD161 + TCRVα7.2 + of CD3 + TCRγδ − cells. Among non-MAIT/non-TCRγδ CD4 + T cells, we identified Treg cells as CD25 + FOXP3 + cells. Finally, the FOXP3-negative T cell population was further divided in the following effector T helper (Th) cells: Th1 as FOXP3 − Tbet + , Th17 as FOXP3 − RORγt + , and Th2 as FOXP3 − CRTH2 + . (B) In vitro expansion of Tbet + Th1, RORγt + Th17, FOXP3 + Treg cells and CRTH2 + Th2 cells in response to gut microbial filtrates of HCs and RRMS patients. PBMCs from a healthy donor were cultured for 72 h in the presence of fecal filtrates derived from HCs or RRMS patients and analyzed by flow cytometry as in (A). Each symbol represents a fecal sample donor ( n = 10 per group). Data are expressed as fold difference over vehicle control (no fecal filtrates). (C) Percentage of in vitro expanded RORγt + Th17 cells in response to gut microbial filtrates of RRMS patients supplemented or not with 10 μM DCA. PBMCs from a healthy donor were cultured for 72 h and subsequently analyzed by flow cytometry ( n = 10 fecal sample donor per group). Bars represent the mean ± SD of data from two independent experiments. All statistical analyses were performed using Mann-Whitney U test to compare the RRMS group with the HC group or the RRMS fecal material (FM) group with the RRMS FM + DCA group. ∗ p < 0.05, ∗∗ p < 0.01; ns, not significant. See also <xref ref-type=Figures S3 and . " width="100%" height="100%">

    Journal: Cell Reports Medicine

    Article Title: Microbiota-produced immune regulatory bile acid metabolites control central nervous system autoimmunity

    doi: 10.1016/j.xcrm.2025.102028

    Figure Lengend Snippet: Alteration of the microbiota-regulated metabolic profile is linked to immune dysregulation in RRMS (A) Percentage of circulating T cell subsets in RRMS patients and HCs measured by multiparametric flow cytometry. γδT cells were identified as CD45 + CD3 + TCRγδ + cells; MAIT cells were defined as CD161 + TCRVα7.2 + of CD3 + TCRγδ − cells. Among non-MAIT/non-TCRγδ CD4 + T cells, we identified Treg cells as CD25 + FOXP3 + cells. Finally, the FOXP3-negative T cell population was further divided in the following effector T helper (Th) cells: Th1 as FOXP3 − Tbet + , Th17 as FOXP3 − RORγt + , and Th2 as FOXP3 − CRTH2 + . (B) In vitro expansion of Tbet + Th1, RORγt + Th17, FOXP3 + Treg cells and CRTH2 + Th2 cells in response to gut microbial filtrates of HCs and RRMS patients. PBMCs from a healthy donor were cultured for 72 h in the presence of fecal filtrates derived from HCs or RRMS patients and analyzed by flow cytometry as in (A). Each symbol represents a fecal sample donor ( n = 10 per group). Data are expressed as fold difference over vehicle control (no fecal filtrates). (C) Percentage of in vitro expanded RORγt + Th17 cells in response to gut microbial filtrates of RRMS patients supplemented or not with 10 μM DCA. PBMCs from a healthy donor were cultured for 72 h and subsequently analyzed by flow cytometry ( n = 10 fecal sample donor per group). Bars represent the mean ± SD of data from two independent experiments. All statistical analyses were performed using Mann-Whitney U test to compare the RRMS group with the HC group or the RRMS fecal material (FM) group with the RRMS FM + DCA group. ∗ p < 0.05, ∗∗ p < 0.01; ns, not significant. See also Figures S3 and .

    Article Snippet: Anti-human CD45 BUV395 , BD Horizon , Cat# 563791; RRID: AB_2744400.

    Techniques: Flow Cytometry, In Vitro, Cell Culture, Derivative Assay, Control, MANN-WHITNEY

    Journal: Cell Reports Medicine

    Article Title: Microbiota-produced immune regulatory bile acid metabolites control central nervous system autoimmunity

    doi: 10.1016/j.xcrm.2025.102028

    Figure Lengend Snippet:

    Article Snippet: Anti-human CD45 BUV395 , BD Horizon , Cat# 563791; RRID: AB_2744400.

    Techniques: Recombinant, Adjuvant, Staining, Activation Assay, Cell Isolation, Software

    List of the markers used in the panel, and their description and purpose

    Journal: STAR Protocols

    Article Title: Protocol for murine multi-tissue deep immunophenotyping using a 40-color full-spectrum flow cytometry panel

    doi: 10.1016/j.xpro.2024.103492

    Figure Lengend Snippet: List of the markers used in the panel, and their description and purpose

    Article Snippet: Anti-mouse CD45 BUV395 (clone 30-F11); 1:5,000 , Thermo Fisher Scientific , Cat# 363-0451-82, RRID: AB_2925264.

    Techniques: Marker, Migration, Glycoproteomics, Activation Assay, Functional Assay

    Intracellular staining mix

    Journal: STAR Protocols

    Article Title: Protocol for murine multi-tissue deep immunophenotyping using a 40-color full-spectrum flow cytometry panel

    doi: 10.1016/j.xpro.2024.103492

    Figure Lengend Snippet: Intracellular staining mix

    Article Snippet: Anti-mouse CD45 BUV395 (clone 30-F11); 1:5,000 , Thermo Fisher Scientific , Cat# 363-0451-82, RRID: AB_2925264.

    Techniques: Staining

    Journal: STAR Protocols

    Article Title: Protocol for murine multi-tissue deep immunophenotyping using a 40-color full-spectrum flow cytometry panel

    doi: 10.1016/j.xpro.2024.103492

    Figure Lengend Snippet:

    Article Snippet: Anti-mouse CD45 BUV395 (clone 30-F11); 1:5,000 , Thermo Fisher Scientific , Cat# 363-0451-82, RRID: AB_2925264.

    Techniques: Purification, Recombinant, Centrifugation, Staining, Software